Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Electron Microscopy Sciences Perfusion Wash, Technical Grade, 7.2-7.4 pH 1000 ML
For all of the best results we offer ready to use Perfusion washes and fixatives which are recommended by the NeuroScience Association. All of our solutions are ready made and are of the highest quality. Perfusion wash and fixative technical grade. To be used for standard stains. Phosphate Buffered Saline 01.M Phosphate Buffer pH 7.2-7.4.
Grade: Technical
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-250.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 250 Grams
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
![Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60040-500.04.JPG-250.jpg)
Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 500 Grams
Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.
It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.
The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.
It is also utilized in the preparation of electrophoresis buffers and PCR reactions.
Sigma Aldrich Fine Chemicals Biosciences Tris-Glycine Buffer 10x Co1L
TRIS Tris-Glycine Buffer 10x Co1L
Chem-Impex International, Inc. Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid, 60-00-4, MFCD00003541, 1KG
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Research Products International Corp PIPES FREE ACID ULTRAPURE, 500GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Rockland Immunochemicals 10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride) (4 x 1 liter) - MB-008-4000
Phosphate buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-25.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 25 Grams
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-500.04.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 500 Grams
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Research Products International Corp MES Sodium Salt 5 KG
MES Sodium Salt, also known as 2-(N-Morpholino)ethanesulfonic acid sodium salt, is a zwitterionic buffer commonly used in biological and biochemical research.
In the life science field, MES Sodium Salt is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 5.5 to 6.7, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MES Sodium Salt is commonly used in protein purification, enzyme assays, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MES Sodium Salt is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes.;
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt solutin500ML
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium calcium manganese(II) or copper(II) ions. HEPES has been classified as Good buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
Research Products International Corp PIPES FREE ACID ULTRAPPURE, 50GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
![Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B75000-1000.0.JPG-250.jpg)
Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 1 Kilogram
Substance Name: bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Formula: C8H19NO5
Molecular Weight:?209
Chemical Purity:?>99%
Soluble in: Water
Store at:?Room Temperature